VICTOR® Nivo™ multimode plate reader 2 | For research use only. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . Read & analyse. Read & analyse. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Plate 25 μL of cells in. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. 5 EXAMPLE OF MAP PLATE . HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Make sure the setup for Eu 3+ Cryptate is used. The CLARIOstar® Plus is our most flexible multi-mode plate reader. FOR HTRF CERTIFIED READER. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Figure 1. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. According to the manufacturers protocol, this protein assay is linear in the range of 0. Plate 25 μL of cells in. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Step 1 Step 2 Step 3 Step 4 Step 5. Specialized filters allow for IR fluorescence. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more. Plate 25 μL of cells in. Read & analyse. FOR HTRF CERTIFIED READER. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Measure elevated and normal expression samples without multiple dilutions. • HTRF®-Certified Reader **. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. For more information about HTRF. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. e. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For the standard curve described, all wells of the microplate are read. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For more information about HTRF. Even if not listed, some. Plus . Revvity. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Table 1. FLUOstar Omega Filter-based multi-mode microplate reader . PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. View Brochure. 1 – 1. Incubate overnight at RT. Highly compatible plates for HTRF. HTRF Setup. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For a list of HTRF-compatible readers and set-up recommendations, please visit . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. For more. If not, our scientific team will help you choose the instrument that best suits your needs and budget. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Make sure the setup for Eu3+ Cryptate is used. Transfer 5 µL of cell supernatant from the 96-well plate to a 384-small volume (SV) white detection plate . For more information. For more information about HTRF. HTRF-specific. 15 / Each of 1; Qty. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. For more. It can also be further streamlinedFOR HTRF CERTIFIED READER. 1. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. Unlike standard ELISA plate readers, MSD. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. Incubate overnight at RT. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. For more information about HTRF. It can also be further streamlined toFOR HTRF CERTIFIED READER. The kit includes everything you need: reagents, buffers and a microplate. No washing steps are required. HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. For more information. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. FOR HTRF CERTIFIED READER. 0. At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. best fluorescence polarization plate reader on the market. 5. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. View Brochure. The laser beam passes through the sample well. Incubate 2h at RT Transfer 16 µL supernatant. The donor and acceptor can be used to label a variety of biomolecules, for. This protocol gives the cells viability and confluence to be monitored. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Plate 25 μL of cells in. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. • HTRF®-Certified Reader **. FOR HTRF CERTIFIED READER. Microplate reader assays requiring infrared (IR) or. Microplate readers are only certified if they. Revvity. For more information. Read & analyse. (donor) of compounds are measured on an HTRF-compatible reader. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. The detection antibody is incubated for 1. If not, our scientific team will help you choose the instrument that best suits your needs and budget. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. 14 seconds for a 384-well plate. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). The optional HTRF-certified filter-based module may be. This protocol gives the cells viability and confluence to be monitored. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The kit includes reagents, buffers and a microplate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Specialized filters allow for IR fluorescence. It can also be further streamlined to a one-. This protocol, HTS designed, allows. FOR HTRF CERTIFIED. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Customer Transformation Hub. com HTRF Microplate Readers. Read & analyse. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. HTRF-compatible SpectraMax Multi-Mode Readers. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. PerkinElmer. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Assay setup for a 384-well low-volume plate. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. This protocol gives the cells viability and confluence to be monitored. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. Envision Nexus. 1. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. Quantify up to 10 analytes per well. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. The signals are produced by, converted by, or transmitted through the. (665 nm and 620 nm) on a compatible HTRF ® reader. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. For more information. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. Incubate 4h at RT. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. VICTOR® Nivo™ multimode plate reader. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. 3. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. NC1656382. Incubate overnight at RT. Incubate 4h at RT. Dual-Luciferase® (DLR™) gene assays. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Filters or monochromators for emission wavelength. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED. Read other about this measurement approach here. For more. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. COMPONENTS ; STORAGE . Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Introduction. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. The lysis buffer 4X is ready-to-use. At Cisbio we provide microplates that help you discover better. It features a modular design that allows users to add or change detection cartridges according to their needs. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Incubate 4h at RT. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. It also integrates with the. Up to 8 decades dynamic range in one measurement with EDR technology. Even if not listed, some readers may be compatible with HTRF technology. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. 4. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Make sure the setup for Eu 3+ Cryptate is used. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. We would like to show you a description here but the site won’t allow us. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Table 1. FOR HTRF CERTIFIED READERThis makes the PHERAstar ®FSX the fastest laser-based TRF reader on the market: 8 seconds for a 96-well plate. EX: HTRF Emission range. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Read & analyse. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. 5 hours before 3 more washing steps. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Plate 25 μL of cells in. Incubate overnight at RT. Endpoint mode experiments measure each well of the microplate only once. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. PHERAstar FSX Powerful and most sensitive HTS plate reader . FOR HTRF CERTIFIED READER. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. • HTRF®-Certified Reader. 96-well culture plateFOR HTRF CERTIFIED READER. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Catalog No. Results for HTRF Reader Control Kit with black and white microplates. Figure 1: Principle of HTRF Cortisol competitive assay. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. -Certified Reader. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. SpectraMax i3/i3x and Paradigm readers can be equipped with. PerkinElmer. Plate 25 μL of cells in. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. NC1286225. It is typically used to determine the concentration of nucleic acids and proteins. Eu cryptate emission level). They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. , filters and/or monochromators), a second optical system for the selection of emission light, and a detector, usually one or multiple photomultiplier tubes (PMTs). Due to the chemical and photophysical. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. In this case the Z´(pronounced Z prime) value comes to mind. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For more information about HTRF ®• HTRF®-Certified Reader. CLARIOstar is equipped with a specific optical device which enables the measurement of. 5 hours before a further 3 washing steps. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Customer Transformation Hub. Using a black. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Transcreener® ADP2 FP assay. Plate 25 μL of cells in. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. For more information about HTRF ®Seal the plate and incubate for Overnight. As shown in Fig. no. 41 mg/ml) was obtained from Thermo Fisher Scientific. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. Dual monochromators for assay flexibility With. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. For a list of HTRF-compatible readers and set-up recommendations, please visit . -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. But sometimes these parameters are not sufficient enough. Each well generally requires 50µL of lysis buffer 1X. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. 6% for 1,408 wells and a Z’ value of 0. The plate is set up for reading and images are acquired according to specified parameters. Figure 3. Incubate 24 hours at RT. For more information about HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. a one- plate assay protocol. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. HTRF is compatible with a large range of available microplate readers. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Make sure the setup for Eu Cryptate is used. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The plate was covered both incubated for one hour at room temperature. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Configure your microplate reader. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . + 4 µL HTRF ® pre-mixed antibodies . HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. They can be used in a no-transfer. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. FOR HTRF CERTIFIED. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The controls are equivalent to the upper and lower limit of. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. This protocol gives the cells viability and confluence to be monitored. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. 55 µL anti-cAMP-Cryptate. • HTRF®-Certified Reader. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. EGFR protein (PV3872, 0. HTRF-specific. DATA REDUCTION & INTERPRETATION 1. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Catalog No. Preconfigured HTRF. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The assay is then ready to be read on an HTRF compatible or certified microplate reader. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. HTRF Microplate Readers. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Provide more sensitive and precise results. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. FOR HTRF CERTIFIED. The donor and acceptor can be used to label a variety of biomolecules, for applications. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. The plate is set up for reading and images are acquired according to specified parameters. Live Viewer for cell-based assays. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. FOR HTRF CERTIFIED READER. The ratio for each well must be calculated individually.